Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_220

Experiment: 40 hrs No Buoyancy Separation

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 8587 and 8588 are separated by 330 nucleotides8588 and 8589 are separated by 482 nucleotides mRNA_219: 8587 - Hypothetical Protein, at 541,631 to 542,423 8587 mRNA_220: 8588 - K11159 K11159 carotenoid cleavage dioxygenase, at 542,754 to 544,832 8588 mRNA_221: 8589 - HMMPfam-Squalene/phytoene synthase-PF00494,ProSitePatterns-Squalene and phytoene synthases signature 2.-PS01045,SUPERFAMILY--SSF48576,TIGRFAM-CarR_dom_SF lycopene cyclase domain-TIGR03462, at 545,315 to 547,571 8589

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Per-strain Table

Position Strand Gene Sysname Fraction 40 hrs No Buoyancy Separation
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542,096 - 8587 mRNA_219 0.59 -0.9
542,213 - 8587 mRNA_219 0.73 -1.5
542,224 - 8587 mRNA_219 0.75 -2.8
542,525 + +0.1
542,699 + -0.7
543,027 + 8588 mRNA_220 0.13 -2.5
543,117 - 8588 mRNA_220 0.17 -1.0
543,126 + 8588 mRNA_220 0.18 +0.0
543,127 + 8588 mRNA_220 0.18 -2.1
543,236 + 8588 mRNA_220 0.23 -2.1
543,421 - 8588 mRNA_220 0.32 -2.0
544,034 + 8588 mRNA_220 0.62 -1.1
544,585 - 8588 mRNA_220 0.88 -2.3
544,586 + 8588 mRNA_220 0.88 +1.4
544,624 - 8588 mRNA_220 0.90 -1.0
544,746 - +0.5
545,045 - -1.8
545,046 - -1.2
545,060 - -0.5
545,071 - -1.0
545,079 + +0.2
545,081 + +0.8
545,082 - -0.6
545,106 + -0.6
545,134 - -2.3
545,182 - -4.1
545,225 - -0.8
545,225 + -1.1
545,231 - -3.6
545,240 + -3.4
545,257 + -1.7
545,380 - -1.0
545,381 + -1.8
545,668 + 8589 mRNA_221 0.16 +0.1

Or see this region's nucleotide sequence