Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_2254

Experiment: 40 hrs Median Buoyancy

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 10621 and 10622 are separated by 227 nucleotides10622 and 10623 are separated by 427 nucleotides mRNA_2253: 10621 - HMMPfam-Staphylococcal nuclease homologue-PF00565,ProSiteProfiles-Thermonuclease domain profile.-PS50830,SMART-Staphylococcal nuclease homologues-SM00318,SUPERFAMILY--SSF50199, at 468,849 to 469,968 10621 mRNA_2254: 10622 - K17822 DCUN1D1_2 DCN1-like protein 1/2, at 470,196 to 471,391 10622 mRNA_2255: 10623 - Hypothetical Protein, at 471,819 to 472,358 10623

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Per-strain Table

Position Strand Gene Sysname Fraction 40 hrs Median Buoyancy
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469,363 - 10621 mRNA_2253 0.46 +0.3
469,372 + 10621 mRNA_2253 0.47 -0.5
469,498 + 10621 mRNA_2253 0.58 -0.1
469,781 - 10621 mRNA_2253 0.83 +0.3
469,791 - 10621 mRNA_2253 0.84 +0.0
469,822 - 10621 mRNA_2253 0.87 +0.5
469,829 - 10621 mRNA_2253 0.88 +0.3
469,957 - +1.2
469,964 - +0.6
469,973 + +0.7
469,980 - +0.1
470,118 + +0.1
470,122 + +1.0
470,233 + +1.9
470,510 + 10622 mRNA_2254 0.26 +0.9
470,547 + 10622 mRNA_2254 0.29 +0.3
470,552 + 10622 mRNA_2254 0.30 +1.8
470,562 - 10622 mRNA_2254 0.31 +0.4
470,673 + 10622 mRNA_2254 0.40 +0.1
471,146 + 10622 mRNA_2254 0.79 -0.7
471,417 - +0.5
471,688 - -0.4
472,305 - -0.9
472,309 - +0.6

Or see this region's nucleotide sequence