Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_53

Experiment: 40 hrs Median Buoyancy

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 8419 and 8420 are separated by 53 nucleotides8420 and 8421 are separated by 210 nucleotides8421 and 8422 are separated by 453 nucleotides mRNA_51: 8419 - K10392 KIF1 kinesin family member 1, at 120,072 to 126,065 8419 mRNA_52: 8420 - Hypothetical Protein, at 126,119 to 126,700 8420 mRNA_53: 8421 - K03014 RPB6, POLR2F DNA-directed RNA polymerases I, II, and III subunit RPABC2, at 126,911 to 127,593 8421 mRNA_54: 8422 - KOG1505 Lysophosphatidic acid acyltransferase LPAAT and related acyltransferases, at 128,047 to 129,356 8422

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Per-strain Table

Position Strand Gene Sysname Fraction 40 hrs Median Buoyancy
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125,964 + +0.4
126,009 - +0.2
126,011 + +1.0
126,151 + -1.7
126,158 + +0.3
126,297 + 8420 mRNA_52 0.31 -0.3
126,298 - 8420 mRNA_52 0.31 -1.7
126,314 + 8420 mRNA_52 0.34 -0.4
126,324 + 8420 mRNA_52 0.35 -0.7
126,324 + 8420 mRNA_52 0.35 -0.2
126,425 - 8420 mRNA_52 0.53 -0.3
126,428 - 8420 mRNA_52 0.53 +1.8
126,430 - 8420 mRNA_52 0.53 +0.3
126,437 - 8420 mRNA_52 0.55 +1.3
126,448 + 8420 mRNA_52 0.57 -0.5
126,452 + 8420 mRNA_52 0.57 +1.1
127,637 + +0.8
127,716 - +2.1
127,733 - +0.5
127,779 - -0.1
127,892 + +0.6
127,908 - -1.0
127,913 + +1.5
128,042 + +0.2
128,185 + 8422 mRNA_54 0.11 +0.5
128,195 + 8422 mRNA_54 0.11 -0.3
128,199 + 8422 mRNA_54 0.12 -0.6
128,340 - 8422 mRNA_54 0.22 +2.5
128,484 - 8422 mRNA_54 0.33 +1.4
128,488 - 8422 mRNA_54 0.34 +0.3

Or see this region's nucleotide sequence