Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_5274

Experiment: Sorted Low BODIPY Gate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 13641 and 13642 are separated by 346 nucleotides13642 and 13643 are separated by 334 nucleotides mRNA_5273: 13641 - K00004 BDH, butB (R,R)-butanediol dehydrogenase / meso-butanediol dehydrogenase / diacetyl reductase, at 332,530 to 334,206 13641 mRNA_5274: 13642 - KOG0032 Ca2+/calmodulin-dependent protein kinase, EF-Hand protein superfamily, at 334,553 to 337,184 13642 mRNA_5275: 13643 - K06688 UBE2C, UBC11 ubiquitin-conjugating enzyme E2 C, at 337,519 to 338,338 13643

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Per-strain Table

Position Strand Gene Sysname Fraction Sorted Low BODIPY Gate
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333,675 - 13641 mRNA_5273 0.68 -0.2
333,689 + 13641 mRNA_5273 0.69 +0.4
334,097 - -0.7
334,292 - -0.2
334,311 - +0.1
334,345 + -0.9
334,383 - -0.9
334,385 + -2.3
334,432 + -0.4
334,574 - +0.2
334,584 + +0.1
334,590 - -3.1
334,732 - -1.3
334,994 - 13642 mRNA_5274 0.17 -1.3
335,365 - 13642 mRNA_5274 0.31 -0.4
335,436 + 13642 mRNA_5274 0.34 -4.1
335,464 + 13642 mRNA_5274 0.35 +0.0
335,609 - 13642 mRNA_5274 0.40 +0.7
336,012 + 13642 mRNA_5274 0.55 -0.5
336,370 + 13642 mRNA_5274 0.69 -1.1
336,769 - 13642 mRNA_5274 0.84 -2.0
336,917 - 13642 mRNA_5274 0.90 -1.0
337,037 + -0.4
337,091 - +0.9
337,101 + -0.1
337,249 + +1.2
337,249 - +0.4
337,252 + +0.6
337,362 + -0.6
337,362 + -0.4
337,365 - -0.3
337,371 - -0.4
337,385 + -1.3
337,496 + -0.6
337,532 + -0.3
337,871 + 13643 mRNA_5275 0.43 +1.7
337,979 - 13643 mRNA_5275 0.56 -0.2
338,097 - 13643 mRNA_5275 0.70 -0.4
338,106 - 13643 mRNA_5275 0.72 +0.1
338,149 + 13643 mRNA_5275 0.77 -1.6

Or see this region's nucleotide sequence