Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_1946

Experiment: Sorted High BODIPY Gate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 10313 and 10314 overlap by 25 nucleotides10314 and 10315 are separated by 264 nucleotides mRNA_1945: 10313 - Hypothetical Protein, at 574,351 to 575,148 10313 mRNA_1946: 10314 - KOG2458 Endoplasmic reticulum protein EP58, contains filamin rod domain and KDEL motif, at 575,124 to 577,080 10314 mRNA_1947: 10315 - K14864 FTSJ1, TRM7 tRNA (cytidine32/guanosine34-2'-O)-methyltransferase, at 577,345 to 578,684 10315

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Per-strain Table

Position Strand Gene Sysname Fraction Sorted High BODIPY Gate
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574,139 - +0.0
574,157 - -2.4
574,163 - -0.4
574,169 - -0.3
574,169 - +0.6
574,172 + -0.1
574,173 + +0.5
574,175 + -2.2
574,178 - -1.0
574,183 + -0.3
574,191 + -1.0
574,193 + +0.2
574,193 + -0.3
574,289 - -2.7
574,384 + -1.8
574,450 - 10313 mRNA_1945 0.12 +0.9
574,459 + 10313 mRNA_1945 0.14 +0.3
574,470 - 10313 mRNA_1945 0.15 +1.8
574,475 + 10313 mRNA_1945 0.16 +0.9
574,510 + 10313 mRNA_1945 0.20 +0.3
574,572 + 10313 mRNA_1945 0.28 -0.6
574,764 - 10313 mRNA_1945 0.52 -1.0
574,803 + 10313 mRNA_1945 0.57 +2.1
575,060 - 10313 mRNA_1945 0.89 -1.6
575,323 - 10314 mRNA_1946 0.10 +1.8
575,481 + 10314 mRNA_1946 0.18 -4.2
575,652 - 10314 mRNA_1946 0.27 +0.2
575,652 + 10314 mRNA_1946 0.27 +0.8
576,687 + 10314 mRNA_1946 0.80 +1.8
576,826 - 10314 mRNA_1946 0.87 -0.3
576,969 + +0.4
576,995 + +0.8
577,010 + +1.1
577,038 - -2.7
577,122 - -0.2
577,176 + -1.4
577,437 + +0.1
577,444 + -0.8
577,548 - 10315 mRNA_1947 0.15 -1.0
577,836 - 10315 mRNA_1947 0.37 +0.1
577,838 + 10315 mRNA_1947 0.37 -1.3
577,851 - 10315 mRNA_1947 0.38 +1.6
577,987 - 10315 mRNA_1947 0.48 +1.1

Or see this region's nucleotide sequence