Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_2246

Experiment: Sorted High BODIPY Gate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 10613 and 10614 are separated by 362 nucleotides10614 and 10615 are separated by 194 nucleotides mRNA_2245: 10613 - K00134 GAPDH, gapA glyceraldehyde 3-phosphate dehydrogenase, at 455,190 to 456,884 10613 mRNA_2246: 10614 - K00026 MDH2 malate dehydrogenase, at 457,247 to 458,914 10614 mRNA_2247: 10615 - Hypothetical Protein, at 459,109 to 460,029 10615

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Per-strain Table

Position Strand Gene Sysname Fraction Sorted High BODIPY Gate
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456,870 - -0.6
456,985 - +0.1
457,022 - -0.5
457,023 - +0.2
457,055 + +0.3
457,062 + -1.3
457,192 - -0.2
457,279 + +0.3
457,307 + +0.4
457,335 - -0.6
457,975 + 10614 mRNA_2246 0.44 -0.9
458,073 - 10614 mRNA_2246 0.50 -0.2
458,203 - 10614 mRNA_2246 0.57 +0.0
458,210 + 10614 mRNA_2246 0.58 -0.3
458,365 + 10614 mRNA_2246 0.67 +0.8
458,367 + 10614 mRNA_2246 0.67 -0.7
458,495 + 10614 mRNA_2246 0.75 -0.6
458,638 - 10614 mRNA_2246 0.83 -0.1
458,654 + 10614 mRNA_2246 0.84 +0.5
458,762 - +0.7
458,791 - +1.4
458,930 - -0.4
459,078 - -0.6
459,085 - -0.6
459,088 - -0.7
459,089 + +0.4
459,092 + +0.5
459,095 - -0.1
459,108 + +0.3
459,200 + -0.4
459,202 - 10615 mRNA_2247 0.10 -0.8
459,210 - 10615 mRNA_2247 0.11 -0.2
459,217 - 10615 mRNA_2247 0.12 +0.6
459,224 - 10615 mRNA_2247 0.12 -1.2
459,233 + 10615 mRNA_2247 0.13 -3.2
459,242 + 10615 mRNA_2247 0.14 -2.7
459,251 + 10615 mRNA_2247 0.15 +0.1
459,384 - 10615 mRNA_2247 0.30 -1.0
459,392 - 10615 mRNA_2247 0.31 -0.6
459,401 - 10615 mRNA_2247 0.32 -0.4
459,573 + 10615 mRNA_2247 0.50 -0.2
459,708 - 10615 mRNA_2247 0.65 -2.1
459,723 + 10615 mRNA_2247 0.67 -1.6
459,772 - 10615 mRNA_2247 0.72 +3.4

Or see this region's nucleotide sequence