Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_254

Experiment: Sorted High BODIPY Gate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 8621 and 8622 are separated by 292 nucleotides8622 and 8623 are separated by 297 nucleotides mRNA_253: 8621 - KOG2273 Membrane coat complex Retromer, subunit VPS5/SNX1, Sorting nexins, and related PX domain-containing proteins, at 611,030 to 612,229 8621 mRNA_254: 8622 - KOG0970 DNA polymerase alpha, catalytic subunit, at 612,522 to 613,328 8622 mRNA_255: 8623 - K02320 POLA1 DNA polymerase alpha subunit A, at 613,626 to 618,274 8623

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Per-strain Table

Position Strand Gene Sysname Fraction Sorted High BODIPY Gate
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611,710 + 8621 mRNA_253 0.57 +0.1
611,712 + 8621 mRNA_253 0.57 +0.6
611,728 + 8621 mRNA_253 0.58 +1.1
611,837 - 8621 mRNA_253 0.67 -1.7
612,247 - +1.7
612,288 - +0.0
612,317 + +0.2

Or see this region's nucleotide sequence