Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_180

Experiment: YNB Oleic Acid

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 8547 and 8548 are separated by 202 nucleotides8548 and 8549 are separated by 595 nucleotides mRNA_179: 8547 - KOG2886 Uncharacterized conserved protein, at 437,893 to 438,740 8547 mRNA_180: 8548 - K01647 CS, gltA citrate synthase, at 438,943 to 441,127 8548 mRNA_181: 8549 - K12870 ISY1 pre-mRNA-splicing factor ISY1, at 441,723 to 442,875 8549

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Per-strain Table

Position Strand Gene Sysname Fraction YNB Oleic Acid
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437,946 - -0.2
437,994 - 8547 mRNA_179 0.12 -2.7
437,995 + 8547 mRNA_179 0.12 +0.9
438,286 + 8547 mRNA_179 0.46 -0.3
438,294 - 8547 mRNA_179 0.47 -0.3
438,468 - 8547 mRNA_179 0.68 +1.2
438,697 + +0.3
438,710 - -0.3
438,711 + -0.3
438,717 - +0.4
438,727 - +0.5
438,734 + -1.9
438,736 - -1.3
438,741 - +1.0
438,751 - +0.0
438,766 - +1.5
438,768 - -1.7
438,862 - +0.7
438,864 - +0.6
438,866 - -1.2
438,890 - +0.1
438,998 + +0.3
439,019 - +0.4
439,019 - -0.8
439,024 - +0.6
439,030 - -0.8
439,048 - -0.7
439,050 + -0.1
439,186 - 8548 mRNA_180 0.11 -0.3
439,206 + 8548 mRNA_180 0.12 -0.6
439,230 + 8548 mRNA_180 0.13 -1.6
439,250 + 8548 mRNA_180 0.14 -0.3
439,332 + 8548 mRNA_180 0.18 +0.2
439,336 + 8548 mRNA_180 0.18 +0.0
439,503 + 8548 mRNA_180 0.26 -0.6
439,598 + 8548 mRNA_180 0.30 +1.8
439,642 - 8548 mRNA_180 0.32 -0.4
439,652 - 8548 mRNA_180 0.32 -0.2
439,778 - 8548 mRNA_180 0.38 -0.4
439,779 + 8548 mRNA_180 0.38 +0.3
439,887 - 8548 mRNA_180 0.43 +0.4
440,229 - 8548 mRNA_180 0.59 -1.1
440,682 - 8548 mRNA_180 0.80 +0.3
440,965 + -0.9
441,072 - +0.1
441,246 - +0.1
441,283 - +0.0
441,611 - +1.8

Or see this region's nucleotide sequence